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Literature summary extracted from

  • Lee, S.K.; Basnet, D.B.; Choi, C.Y.; Sohng, J.K.; Ahn, J.S.; Yoon, Y.J.
    The role of erythromycin C-12 hydroxylase, EryK, as a substitute for PikC hydroxylase in pikromycin biosynthesis (2004), Bioorg. Chem., 32, 549-559.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.33 expression in a Streptomyces venezuelae pikC mutant Saccharopolyspora erythraea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.33 molecular modeling of the active site. For substrate narbomycin, the hydrophobic residues Ala223, Leu224, Leu225, and Leu226 may form hydrophobic interactions with the methyl group at C-6 and C-8 including C-7 of the macrolide ring Saccharopolyspora erythraea

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.154 Saccharopolyspora erythraea P48635
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1.14.15.33 Saccharopolyspora erythraea P48635 erythromycin C-12 hydroxylase is able to substitute for pikromycin hydroxylase which is responsible for the hydroxylation of 10-deoxymethymycin and narbomycin. Erythromycin C-12 hydroxylase accepts both 12- and 14-membered macrolides
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.154 additional information in a Saccharopolyspora venezuelae pikC deletion mutant, EryK can catalyze the hydroxylation of YC-17 and narbomycin to generate methymycin/neomethymycin and pikromycin, respectively. EryK is flexible toward some alternative polyketides and can be useful for structural diversification of macrolides by post-polyketide synthase hydroxylation Saccharopolyspora erythraea ?
-
 ?
1.14.15.33 10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
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 Saccharopolyspora erythraea methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.33 10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.33 additional information erythromycin C-12 hydroxylase is able to substitute for pikromycin hydroxylase which is responsible for the hydroxylation of 10-deoxymethymycin and narbomycin. Erythromycin C-12 hydroxylase accepts both 12- and 14-membered macrolides Saccharopolyspora erythraea ?
-
 ?
1.14.15.33 narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
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 Saccharopolyspora erythraea pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.154 EryK
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 Saccharopolyspora erythraea
1.14.15.33 erythromycin C-12 hydroxylase
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 Saccharopolyspora erythraea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.154 cytochrome P-450 a heme-thiolate protein (P-450) Saccharopolyspora erythraea